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Assessing actual nutrient digestibility is key for balanced rations

Clayton Stoffel for Progressive Dairy Published on 25 May 2022

Given this year’s uncertainties surrounding fertilizer, fuel and freight, one thing is certain – the feed bill will be going up. To prepare for a scenario where ingredients are expensive and potentially hard to come by, many producers and nutritionists are taking a hard look at rations and evaluating what can be removed.

Some may opt to pull back on purchased feeds like soyhulls in favor of homegrown alfalfa silage or switch from steam-flaked corn to dry ground corn. While this strategy focuses heavily on cost, a more effective way to maximize the value of the total mixed ration (TMR) is to prioritize nutrient digestibility and ensure cows are making the most of every bite.



Apparent total tract digestibility (total tract digestibility for short) is one on-farm measurement that can be used to benchmark a dairy against themselves and their peers over time and evaluate the impact of feed changes.

What is total tract digestibility?

Total tract digestibility estimates the absorption of a nutrient through the digestive tract. It is often applied to nutrients such as neutral detergent fiber (NDF) and starch, but it can also be used to assess other nutrients including protein, fat and organic matter.

The analysis is conducted by collecting TMR and fecal samples from the cows in question and analyzing them both for the nutrient of interest and a marker. Commonly used markers are lignin and, more recently, undigestible NDF (uNDF 240 or uNDF 120). Knowing the concentration of the marker in the feed and feces is important because it establishes a relationship between intake and excretion. We can then use this relationship to account for intake and excretion without knowing the exact volume of feed consumed or manure produced. The equation to calculate total tract digestibility (in this example, starch) is:

Total tract starch digestibility = 100% - [(TMR uNDF120 % / fecal uNDF120 %) x (fecal starch % / TMR starch %)]

Total tract digestibility does have its limitations. Applying it to nutrients such as fat and protein can be more challenging than starch and NDF because cows can synthesize these nutrients in their bodies. What is excreted in the manure may come from both the feed and what is synthesized in the cow. This would result in digestibility being underestimated. Additionally, it is difficult to sample TMRs accurately.


Collecting multiple representative samples is recommended to help alleviate some of this sampling error. The turnaround on this analysis can also be lengthy due to the required incubation time (up to 10 days) for the uNDF marker. The time issue could be solved by utilizing near-infrared (NIR) analysis. However, some researchers have shown poor correlations between NIR and benchtop chemistry measurements of total tract digestibility. Caution should be taken when using NIR analysis to calculate total tract digestibility.

Despite the challenges associated with total tract digestibility, research has shown strong relationships between on-farm measured total tract organic matter digestibility and energy-corrected milk yield. We can also calculate the impact improved total tract digestibility should have on production: Each percentage unit increase in total tract starch digestibility provides enough energy for approximately 0.7 pound of milk, and a 2 to 3 percentage unit increase in NDF digestibility provides enough energy for 1 pound of milk.

On-farm examples of use

Over the last several months, our company has pioneered an analysis which uses total tract digestibility measurements to help producers benchmark their herd against the rest of the industry. To date, 18 herds representing over 70,000 cows across the country have been sampled. The program uses data from Rock River Laboratory to set average and goal benchmarks for both starch and NDF digestibility.

Initial results show the farms sampled are above-average on both starch and NDF digestibility, with 78% of farms having total tract starch digestibilities greater than the historical average of 94.61% and 67% of farms with total tract NDF digestibilities greater than the historical average of 37.45%. With that said, there is still room for improvement. Less than 25% of farms hit the goal for either starch (97.6%) or NDF (46.8%) digestibility.

In addition to benchmarking herds, total tract digestibility can be used to monitor the effects of dietary changes or additions. When products designed to enhance rumen function or support gut health are used, it can be difficult to see changes in milk or component yield amid the day-to-day variations on-farm. Assuming the product was effective, this can be due to the fact that cows partition nutrients where they are needed. Instead of using increased nutrients or energy to improve productivity, a cow may shuttle them toward reproduction, immune function or growth and body condition. Total tract digestibility allows us to peek inside the cow to see if a product or ingredient had an upstream effect on nutrient availability.

Our company has used this tool quite effectively when evaluating probiotic products on-farm. As shown in Figure 1 feeding a probiotic resulted in a very measurable and consistent response in total tract starch digestibility, which is dependent on the initial total tract starch digestibility.


Increase in total tract starch digestibility

In these times of rising feed costs and decreased feed availability, it is vital to have confidence in all ingredients and products used in the TMR. Understanding nutrient digestibility can provide this assurance and be leveraged to help cows get as much value as possible out of each bite of feed. Strategically monitoring total tract digestibility of the TMR can be a valuable tool in accomplishing this goal.  end mark

Clayton Stoffel
  • Clayton Stoffel

  • Technical Services Manager
  • Papillon
  • Email Clayton Stoffel